Part:BBa_K864204:Design

OmpF

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 99
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 232
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Illegal PstI restriction site removed by silent mutations T>A at position 66 and A>T at position 69 using primers listed below (annealing sequences are underlined)

ompF_rsr_F CTGAAATCTATAACAAAGATGGCAA

ompF_rsr_R CTGCGTTTGCAGTACCTGCT

Source

Amplified from MG1655 (accession NC_000913) chromosome using primers listed below (annealing sequences are underlined)

ompF_BB_F TTCGAATTCGCGGCCGCTTCTAGATGATGAAGCGCAATATTC

ompF_BB_R CGGCCGCTACTAGTATTATTAGAACTGGTAAACGATACC